Plenary Lecture

Plenary Lecture

Non-Invasive Expressions of ipt in Whole Plants or Roots
Indicate Cytokinins are Synthesized in Plant Aerial Parts, and Coordinate with Light Affect on the Phenotypes and the Formation of Anthocyanins, Lignins

Professor Jian-Chun Guo
Key Laboratory of Tropical Crops Biotechnology Agriculture Ministry
Institute of Tropical Bioscience and Biotechnology
Chinese Academy of Tropical Agricultural Sciences
571101 Haikou, China

To study the plant roots how in response to ipt gene activation, the transcriptional fusions of ipt-GUS and GUS-ipt were expressed in roots, or in whole plants of Arabidopsis under the control of a root-specific promoter TobRT7, or a CaMV35S promoter through pOp/LhG4 system in non-invasive conditions. The transgenic plants with constitutive expression of ipt-GUS or GUS-ipt showed 15-25 fold, or 1-2 fold increased cytokinin levels, respectively. ipt-GUS expressing Arabidopsis had severe root inhibition, serrated leaves, no or few sterile flowers, and an enlarged and retard phenotypes. The seedlings of enlarged phenotype had an enlarged shoot and shoot apical meristem. The ratio of the two phenotypes related with sucrose content in medium and light intensity. While, GUS-ipt expressing Arabidopsis grew faster, flowered early, and had more lateral shoots. However, when ipt-GUS and GUS-ipt were specially expressed in roots under the control of TobRT7, neither cytokinin content in roots or shoots, nor phenotypes were altered. In cytokinin over-producing ipt-GUS expressing Arabidopsis, light and aerial parts of plants played an important role for cytokinin synthesis and root inhibition, and ipt gene was vigorously expressed at the shoot apical parts. Meanwhile, calli were induced at the shoot apical parts of some cytokinin over-producing ipt-GUS expressing Arabidopsis. Increased cytokinins in ipt:::GUS activated Arabidopsis induced the syntheses of anthocyanins and lignins, while light speeded up the anthocyanin accumulation. The endogenous increased cytokinins and exogenous addition of sucrose in MS medium caused the similar effects on the formation of anthocyanins and lignins in shoot, especially in shoot apical meristem. Cytokinins induced CHS transcription in ipt:::GUS activated Arabidopsis seedlings in vigorous vegetative growth period, in which the transcription of CHS was low and photosynthesis was vigorous in wild type Columbia. Cytokinins were capable of influencing either the syntheses or distribution of chlorophylls, but did not alter cab and rbcS transcriptions in light-grown seedlings.

Brief Biography of the Speaker:
GUO Jianchun: Female, 1965, Chinese, Professor. Molecular Genetics PH.D of Masaryk University in Cezch. Supervisor of PH.D in the areas of Crop Genetic Breeding; Biochemistry and Molecular Biology; Plant Molecular Genetics. Deputy Secretary-General of Hainan Bioengineering Association in China. Works on Molecular Biology and Plant Engineering. Researches on Plant Physiology, the Mechanism of Plant Salt Tolerance, and the Engineering of Adversity Tolerance in Tropical Crops.

WSEAS Unifying the Science